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human il6 duoset elisa kit  (R&D Systems)


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    Structured Review

    R&D Systems human il6 duoset elisa kit
    Human Il6 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il6+duoset+elisa+kit/Human+IL-6+DuoSet+ELISA/us12583917-711-15-23
    Average 96 stars, based on 1183 article reviews
    human il6 duoset elisa kit - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: oHSV-P10 reduces glioma stem cell enrichment after oncolytic HSV therapy
    Article Snippet: Image analysis was done using ImageJ software (NIH). .. IL6 ELSIA from culture supernatant 48 hpi at an MOI of 0.005 was performed using Human IL6 DuoSet ELISA kit from R & D Systems (Minneapolis, MN) (Cat. No. DY206) as per the manufacturer’s instructions. ..

    Article Title: IL17A antibodies and antagonists for veterinary use
    Article Snippet: .. The amount of IL6 secreted from the cells into the medium was measured by a Human IL6 DUOSET ELISA kit (Catalog No. DY206-05, R&D Systems). ..

    Article Title: IL17A antibodies and antagonists for veterinary use
    Article Snippet: .. The amount of IL6 secreted from the cells into the medium was measured by a Human IL6 DuoSet ELISA kit (Catalog No. DY206-05, R&D Systems). ..

    Article Title: Intratumoral acidosis fosters cancer-induced bone pain through the activation of the mesenchymal tumor-associated stroma in bone metastasis from breast carcinoma
    Article Snippet: .. Unless otherwise indicated, all reagents were obtained from Sigma: IMDM, penicillin, streptomycin, Trizol, unbuffered RPMI-1640 (Life Technologies); anti-fibroblast magnetic microbeads (Miltenyi Biotech); FBS, DMEM (Euroclone); glucose (Merk); MuLV Reverse Transcriptase (Applied Biosystems); RANKL, M-CSF (Peprotech); nitrocellulose membrane, D-luciferin (Thermo Fisher Scientific); BCA protein assay, Restore Western Blot Stripping buffer, Pierce ECL 2 Western Blotting Substrate (Pierce); TruSeq RNA Sample Prep Kit v2, Cycle Sequencing v4 regents (Illumina); Human IL6 DuoSet ELISA Kit, Human CXCL8/IL8 Quantikine ELISA Kit, Human Free BDNF Quantikine ELISA kit (R&D Systems Inc.). .. The antibodies used were: anti-rabbit Alexafluor, anti-mouse Alexafluor (Molecular Probes); Anti-MCT4 (sc-50329), anti-TBP (sc-204, Santa Cruz Biotechnology); anti-cytokeratin (M0821, Dako); anti-cytokeratin-TRITC (41-9003, Affimetrix eBioscience); anti-LAMP2 (HPA029100); anti-IL8 (AB18672, Abcam); anti-vimentin (sc-6260, Santa Cruz Biotechnology); omeprazole (Sandoz); ketamine (MSD Animal Health Srl).

    Reverse Transcription:

    Article Title: Intratumoral acidosis fosters cancer-induced bone pain through the activation of the mesenchymal tumor-associated stroma in bone metastasis from breast carcinoma
    Article Snippet: .. Unless otherwise indicated, all reagents were obtained from Sigma: IMDM, penicillin, streptomycin, Trizol, unbuffered RPMI-1640 (Life Technologies); anti-fibroblast magnetic microbeads (Miltenyi Biotech); FBS, DMEM (Euroclone); glucose (Merk); MuLV Reverse Transcriptase (Applied Biosystems); RANKL, M-CSF (Peprotech); nitrocellulose membrane, D-luciferin (Thermo Fisher Scientific); BCA protein assay, Restore Western Blot Stripping buffer, Pierce ECL 2 Western Blotting Substrate (Pierce); TruSeq RNA Sample Prep Kit v2, Cycle Sequencing v4 regents (Illumina); Human IL6 DuoSet ELISA Kit, Human CXCL8/IL8 Quantikine ELISA Kit, Human Free BDNF Quantikine ELISA kit (R&D Systems Inc.). .. The antibodies used were: anti-rabbit Alexafluor, anti-mouse Alexafluor (Molecular Probes); Anti-MCT4 (sc-50329), anti-TBP (sc-204, Santa Cruz Biotechnology); anti-cytokeratin (M0821, Dako); anti-cytokeratin-TRITC (41-9003, Affimetrix eBioscience); anti-LAMP2 (HPA029100); anti-IL8 (AB18672, Abcam); anti-vimentin (sc-6260, Santa Cruz Biotechnology); omeprazole (Sandoz); ketamine (MSD Animal Health Srl).

    Membrane:

    Article Title: Intratumoral acidosis fosters cancer-induced bone pain through the activation of the mesenchymal tumor-associated stroma in bone metastasis from breast carcinoma
    Article Snippet: .. Unless otherwise indicated, all reagents were obtained from Sigma: IMDM, penicillin, streptomycin, Trizol, unbuffered RPMI-1640 (Life Technologies); anti-fibroblast magnetic microbeads (Miltenyi Biotech); FBS, DMEM (Euroclone); glucose (Merk); MuLV Reverse Transcriptase (Applied Biosystems); RANKL, M-CSF (Peprotech); nitrocellulose membrane, D-luciferin (Thermo Fisher Scientific); BCA protein assay, Restore Western Blot Stripping buffer, Pierce ECL 2 Western Blotting Substrate (Pierce); TruSeq RNA Sample Prep Kit v2, Cycle Sequencing v4 regents (Illumina); Human IL6 DuoSet ELISA Kit, Human CXCL8/IL8 Quantikine ELISA Kit, Human Free BDNF Quantikine ELISA kit (R&D Systems Inc.). .. The antibodies used were: anti-rabbit Alexafluor, anti-mouse Alexafluor (Molecular Probes); Anti-MCT4 (sc-50329), anti-TBP (sc-204, Santa Cruz Biotechnology); anti-cytokeratin (M0821, Dako); anti-cytokeratin-TRITC (41-9003, Affimetrix eBioscience); anti-LAMP2 (HPA029100); anti-IL8 (AB18672, Abcam); anti-vimentin (sc-6260, Santa Cruz Biotechnology); omeprazole (Sandoz); ketamine (MSD Animal Health Srl).

    Bicinchoninic Acid Protein Assay:

    Article Title: Intratumoral acidosis fosters cancer-induced bone pain through the activation of the mesenchymal tumor-associated stroma in bone metastasis from breast carcinoma
    Article Snippet: .. Unless otherwise indicated, all reagents were obtained from Sigma: IMDM, penicillin, streptomycin, Trizol, unbuffered RPMI-1640 (Life Technologies); anti-fibroblast magnetic microbeads (Miltenyi Biotech); FBS, DMEM (Euroclone); glucose (Merk); MuLV Reverse Transcriptase (Applied Biosystems); RANKL, M-CSF (Peprotech); nitrocellulose membrane, D-luciferin (Thermo Fisher Scientific); BCA protein assay, Restore Western Blot Stripping buffer, Pierce ECL 2 Western Blotting Substrate (Pierce); TruSeq RNA Sample Prep Kit v2, Cycle Sequencing v4 regents (Illumina); Human IL6 DuoSet ELISA Kit, Human CXCL8/IL8 Quantikine ELISA Kit, Human Free BDNF Quantikine ELISA kit (R&D Systems Inc.). .. The antibodies used were: anti-rabbit Alexafluor, anti-mouse Alexafluor (Molecular Probes); Anti-MCT4 (sc-50329), anti-TBP (sc-204, Santa Cruz Biotechnology); anti-cytokeratin (M0821, Dako); anti-cytokeratin-TRITC (41-9003, Affimetrix eBioscience); anti-LAMP2 (HPA029100); anti-IL8 (AB18672, Abcam); anti-vimentin (sc-6260, Santa Cruz Biotechnology); omeprazole (Sandoz); ketamine (MSD Animal Health Srl).

    Western Blot:

    Article Title: Intratumoral acidosis fosters cancer-induced bone pain through the activation of the mesenchymal tumor-associated stroma in bone metastasis from breast carcinoma
    Article Snippet: .. Unless otherwise indicated, all reagents were obtained from Sigma: IMDM, penicillin, streptomycin, Trizol, unbuffered RPMI-1640 (Life Technologies); anti-fibroblast magnetic microbeads (Miltenyi Biotech); FBS, DMEM (Euroclone); glucose (Merk); MuLV Reverse Transcriptase (Applied Biosystems); RANKL, M-CSF (Peprotech); nitrocellulose membrane, D-luciferin (Thermo Fisher Scientific); BCA protein assay, Restore Western Blot Stripping buffer, Pierce ECL 2 Western Blotting Substrate (Pierce); TruSeq RNA Sample Prep Kit v2, Cycle Sequencing v4 regents (Illumina); Human IL6 DuoSet ELISA Kit, Human CXCL8/IL8 Quantikine ELISA Kit, Human Free BDNF Quantikine ELISA kit (R&D Systems Inc.). .. The antibodies used were: anti-rabbit Alexafluor, anti-mouse Alexafluor (Molecular Probes); Anti-MCT4 (sc-50329), anti-TBP (sc-204, Santa Cruz Biotechnology); anti-cytokeratin (M0821, Dako); anti-cytokeratin-TRITC (41-9003, Affimetrix eBioscience); anti-LAMP2 (HPA029100); anti-IL8 (AB18672, Abcam); anti-vimentin (sc-6260, Santa Cruz Biotechnology); omeprazole (Sandoz); ketamine (MSD Animal Health Srl).

    Stripping:

    Article Title: Intratumoral acidosis fosters cancer-induced bone pain through the activation of the mesenchymal tumor-associated stroma in bone metastasis from breast carcinoma
    Article Snippet: .. Unless otherwise indicated, all reagents were obtained from Sigma: IMDM, penicillin, streptomycin, Trizol, unbuffered RPMI-1640 (Life Technologies); anti-fibroblast magnetic microbeads (Miltenyi Biotech); FBS, DMEM (Euroclone); glucose (Merk); MuLV Reverse Transcriptase (Applied Biosystems); RANKL, M-CSF (Peprotech); nitrocellulose membrane, D-luciferin (Thermo Fisher Scientific); BCA protein assay, Restore Western Blot Stripping buffer, Pierce ECL 2 Western Blotting Substrate (Pierce); TruSeq RNA Sample Prep Kit v2, Cycle Sequencing v4 regents (Illumina); Human IL6 DuoSet ELISA Kit, Human CXCL8/IL8 Quantikine ELISA Kit, Human Free BDNF Quantikine ELISA kit (R&D Systems Inc.). .. The antibodies used were: anti-rabbit Alexafluor, anti-mouse Alexafluor (Molecular Probes); Anti-MCT4 (sc-50329), anti-TBP (sc-204, Santa Cruz Biotechnology); anti-cytokeratin (M0821, Dako); anti-cytokeratin-TRITC (41-9003, Affimetrix eBioscience); anti-LAMP2 (HPA029100); anti-IL8 (AB18672, Abcam); anti-vimentin (sc-6260, Santa Cruz Biotechnology); omeprazole (Sandoz); ketamine (MSD Animal Health Srl).

    Sample Prep:

    Article Title: Intratumoral acidosis fosters cancer-induced bone pain through the activation of the mesenchymal tumor-associated stroma in bone metastasis from breast carcinoma
    Article Snippet: .. Unless otherwise indicated, all reagents were obtained from Sigma: IMDM, penicillin, streptomycin, Trizol, unbuffered RPMI-1640 (Life Technologies); anti-fibroblast magnetic microbeads (Miltenyi Biotech); FBS, DMEM (Euroclone); glucose (Merk); MuLV Reverse Transcriptase (Applied Biosystems); RANKL, M-CSF (Peprotech); nitrocellulose membrane, D-luciferin (Thermo Fisher Scientific); BCA protein assay, Restore Western Blot Stripping buffer, Pierce ECL 2 Western Blotting Substrate (Pierce); TruSeq RNA Sample Prep Kit v2, Cycle Sequencing v4 regents (Illumina); Human IL6 DuoSet ELISA Kit, Human CXCL8/IL8 Quantikine ELISA Kit, Human Free BDNF Quantikine ELISA kit (R&D Systems Inc.). .. The antibodies used were: anti-rabbit Alexafluor, anti-mouse Alexafluor (Molecular Probes); Anti-MCT4 (sc-50329), anti-TBP (sc-204, Santa Cruz Biotechnology); anti-cytokeratin (M0821, Dako); anti-cytokeratin-TRITC (41-9003, Affimetrix eBioscience); anti-LAMP2 (HPA029100); anti-IL8 (AB18672, Abcam); anti-vimentin (sc-6260, Santa Cruz Biotechnology); omeprazole (Sandoz); ketamine (MSD Animal Health Srl).

    Sequencing:

    Article Title: Intratumoral acidosis fosters cancer-induced bone pain through the activation of the mesenchymal tumor-associated stroma in bone metastasis from breast carcinoma
    Article Snippet: .. Unless otherwise indicated, all reagents were obtained from Sigma: IMDM, penicillin, streptomycin, Trizol, unbuffered RPMI-1640 (Life Technologies); anti-fibroblast magnetic microbeads (Miltenyi Biotech); FBS, DMEM (Euroclone); glucose (Merk); MuLV Reverse Transcriptase (Applied Biosystems); RANKL, M-CSF (Peprotech); nitrocellulose membrane, D-luciferin (Thermo Fisher Scientific); BCA protein assay, Restore Western Blot Stripping buffer, Pierce ECL 2 Western Blotting Substrate (Pierce); TruSeq RNA Sample Prep Kit v2, Cycle Sequencing v4 regents (Illumina); Human IL6 DuoSet ELISA Kit, Human CXCL8/IL8 Quantikine ELISA Kit, Human Free BDNF Quantikine ELISA kit (R&D Systems Inc.). .. The antibodies used were: anti-rabbit Alexafluor, anti-mouse Alexafluor (Molecular Probes); Anti-MCT4 (sc-50329), anti-TBP (sc-204, Santa Cruz Biotechnology); anti-cytokeratin (M0821, Dako); anti-cytokeratin-TRITC (41-9003, Affimetrix eBioscience); anti-LAMP2 (HPA029100); anti-IL8 (AB18672, Abcam); anti-vimentin (sc-6260, Santa Cruz Biotechnology); omeprazole (Sandoz); ketamine (MSD Animal Health Srl).



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    a Scatter dot plot of pathway enrichment analysis of genes significantly changed after HOTAIR silencing (FDR < 0.05; Log fold change +/− 1). Blue dots: significantly enriched pathways, darker color corresponds to lower p -values. Grey dots represent pathways with p > 0.05. Fisher exact test. b Expression of COL1A1 ( n = 8), COL1A2 ( n = 6) and COL1A3 ( n = 5) measured by quantitative PCR between SF transfected with control (GapCTRL) or HOTAIR targeting GapmeR (GapHOTAIR) after 48 h. Control transfected cells were set to 1. Mean +/− standard deviation is shown. c Pro-Collagen was measured in supernatants of SF transfected with control or HOTAIR targeting GapmeR after 48 h ( n = 10) and 72 h ( n = 11) by <t>ELISA.</t> d Collagen I was stained by immunohistochemistry in 3D micromasses formed with control or HOTAIR silenced SF ( n = 6). Right panel: representative pictures, 25× magnification, scale bare = 200 µm; left panel: quantification with ImageJ and analysis with paired t test. e Expression of FGF2 ( n = 7), FGF7 ( n = 8 ) , FGF8 ( n = 7 ) and FGFR2 ( n = 8) measured by quantitative PCR between SF transfected with control or HOTAIR targeting GapmeR after 48 h. Control transfected cells were set to 1. Mean +/− standard deviation is shown. One sample t test. f FGF8 secretion in cell culture supernatants 48 h ( n = 11) and 72 h ( n = 5) after transfection measured by ELISA. Paired t test. g Expression of AKT and phosphorylated AKT in SF transfected with control or HOTAIR targeting GapmeR after 48 h ( n = 4) and 72 h ( n = 10). Right panel: representative examples; left panel: densitometric analysis. Paired t test.
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    Image Search Results


    a Scatter dot plot of pathway enrichment analysis of genes significantly changed after HOTAIR silencing (FDR < 0.05; Log fold change +/− 1). Blue dots: significantly enriched pathways, darker color corresponds to lower p -values. Grey dots represent pathways with p > 0.05. Fisher exact test. b Expression of COL1A1 ( n = 8), COL1A2 ( n = 6) and COL1A3 ( n = 5) measured by quantitative PCR between SF transfected with control (GapCTRL) or HOTAIR targeting GapmeR (GapHOTAIR) after 48 h. Control transfected cells were set to 1. Mean +/− standard deviation is shown. c Pro-Collagen was measured in supernatants of SF transfected with control or HOTAIR targeting GapmeR after 48 h ( n = 10) and 72 h ( n = 11) by ELISA. d Collagen I was stained by immunohistochemistry in 3D micromasses formed with control or HOTAIR silenced SF ( n = 6). Right panel: representative pictures, 25× magnification, scale bare = 200 µm; left panel: quantification with ImageJ and analysis with paired t test. e Expression of FGF2 ( n = 7), FGF7 ( n = 8 ) , FGF8 ( n = 7 ) and FGFR2 ( n = 8) measured by quantitative PCR between SF transfected with control or HOTAIR targeting GapmeR after 48 h. Control transfected cells were set to 1. Mean +/− standard deviation is shown. One sample t test. f FGF8 secretion in cell culture supernatants 48 h ( n = 11) and 72 h ( n = 5) after transfection measured by ELISA. Paired t test. g Expression of AKT and phosphorylated AKT in SF transfected with control or HOTAIR targeting GapmeR after 48 h ( n = 4) and 72 h ( n = 10). Right panel: representative examples; left panel: densitometric analysis. Paired t test.

    Journal: Nature Communications

    Article Title: The long non-coding RNA HOTAIR contributes to joint-specific gene expression in rheumatoid arthritis

    doi: 10.1038/s41467-023-44053-w

    Figure Lengend Snippet: a Scatter dot plot of pathway enrichment analysis of genes significantly changed after HOTAIR silencing (FDR < 0.05; Log fold change +/− 1). Blue dots: significantly enriched pathways, darker color corresponds to lower p -values. Grey dots represent pathways with p > 0.05. Fisher exact test. b Expression of COL1A1 ( n = 8), COL1A2 ( n = 6) and COL1A3 ( n = 5) measured by quantitative PCR between SF transfected with control (GapCTRL) or HOTAIR targeting GapmeR (GapHOTAIR) after 48 h. Control transfected cells were set to 1. Mean +/− standard deviation is shown. c Pro-Collagen was measured in supernatants of SF transfected with control or HOTAIR targeting GapmeR after 48 h ( n = 10) and 72 h ( n = 11) by ELISA. d Collagen I was stained by immunohistochemistry in 3D micromasses formed with control or HOTAIR silenced SF ( n = 6). Right panel: representative pictures, 25× magnification, scale bare = 200 µm; left panel: quantification with ImageJ and analysis with paired t test. e Expression of FGF2 ( n = 7), FGF7 ( n = 8 ) , FGF8 ( n = 7 ) and FGFR2 ( n = 8) measured by quantitative PCR between SF transfected with control or HOTAIR targeting GapmeR after 48 h. Control transfected cells were set to 1. Mean +/− standard deviation is shown. One sample t test. f FGF8 secretion in cell culture supernatants 48 h ( n = 11) and 72 h ( n = 5) after transfection measured by ELISA. Paired t test. g Expression of AKT and phosphorylated AKT in SF transfected with control or HOTAIR targeting GapmeR after 48 h ( n = 4) and 72 h ( n = 10). Right panel: representative examples; left panel: densitometric analysis. Paired t test.

    Article Snippet: The human CXCL12 DuoSet ELISA kit (R&D Systems), the human IL12-p35 ELISA kit (Elabscience), the human IL6 ELISA DuoSet kit (R&D Systems), the human procollagen 1α ELISA Set (BD Biosciences), and the human FGF8 ELISA Kit (MyBioSource), respectively was used with cell culture supernatants.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection, Control, Standard Deviation, Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry, Cell Culture

    a Expression of CTNNB1 ( n = 8), LRP6 ( n = 5), LGR5 ( n = 7) and GSK3B ( n = 9 ) measured by quantitative PCR between synovial fibroblasts (SF) transfected with control (GapCTRL) or HOTAIR targeting GapmeR (GapHOTAIR) after 48 h. Control transfected cells were set to 1, Mean +/− standard deviation is shown, one sample t test. b Activation of the canonical Wnt pathway was assessed by luciferase assay with a TCF/LEF reporter assay ( n = 6) or ( c ) with a Wnt reporter gene (Top) or a mutated Wnt reported gene (Fop) as control ( n = 5) in SF transfected with control or HOTAIR targeting GapmeR after 48 h.Wilcoxon and one sample t test, respectively. d Expression of IL12A ( n = 6), IL6 ( n = 6), CXCL12 ( n = 10), PTEN ( n = 7), FOXO1 ( n = 7) and RUNX1 ( n = 6) measured by quantitative PCR between SF transfected with control or HOTAIR targeting GapmeR after 48 h. Control transfected cells were set to 1. Mean +/− standard deviation is shown. One sample t test. e IL12p35 (48 h: n = 9, 72 h: n = 7), ( f ) IL-6 (48 h: n = 13, 72 h: n = 12) and ( g ) CXCL12 (48 h: n = 8, 72 h: n = 9) were measured in supernatants of SF transfected with control or HOTAIR targeting GapmeR after 48 h and 72 h by ELISA. All paired t test. h IL-6 was measured in supernatants of micromasses after 7 days by ELISA ( n = 7). Paired t test. Not reported p -values on the figure were not significant.

    Journal: Nature Communications

    Article Title: The long non-coding RNA HOTAIR contributes to joint-specific gene expression in rheumatoid arthritis

    doi: 10.1038/s41467-023-44053-w

    Figure Lengend Snippet: a Expression of CTNNB1 ( n = 8), LRP6 ( n = 5), LGR5 ( n = 7) and GSK3B ( n = 9 ) measured by quantitative PCR between synovial fibroblasts (SF) transfected with control (GapCTRL) or HOTAIR targeting GapmeR (GapHOTAIR) after 48 h. Control transfected cells were set to 1, Mean +/− standard deviation is shown, one sample t test. b Activation of the canonical Wnt pathway was assessed by luciferase assay with a TCF/LEF reporter assay ( n = 6) or ( c ) with a Wnt reporter gene (Top) or a mutated Wnt reported gene (Fop) as control ( n = 5) in SF transfected with control or HOTAIR targeting GapmeR after 48 h.Wilcoxon and one sample t test, respectively. d Expression of IL12A ( n = 6), IL6 ( n = 6), CXCL12 ( n = 10), PTEN ( n = 7), FOXO1 ( n = 7) and RUNX1 ( n = 6) measured by quantitative PCR between SF transfected with control or HOTAIR targeting GapmeR after 48 h. Control transfected cells were set to 1. Mean +/− standard deviation is shown. One sample t test. e IL12p35 (48 h: n = 9, 72 h: n = 7), ( f ) IL-6 (48 h: n = 13, 72 h: n = 12) and ( g ) CXCL12 (48 h: n = 8, 72 h: n = 9) were measured in supernatants of SF transfected with control or HOTAIR targeting GapmeR after 48 h and 72 h by ELISA. All paired t test. h IL-6 was measured in supernatants of micromasses after 7 days by ELISA ( n = 7). Paired t test. Not reported p -values on the figure were not significant.

    Article Snippet: The human CXCL12 DuoSet ELISA kit (R&D Systems), the human IL12-p35 ELISA kit (Elabscience), the human IL6 ELISA DuoSet kit (R&D Systems), the human procollagen 1α ELISA Set (BD Biosciences), and the human FGF8 ELISA Kit (MyBioSource), respectively was used with cell culture supernatants.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection, Control, Standard Deviation, Activation Assay, Luciferase, Reporter Assay, Enzyme-linked Immunosorbent Assay

    Fig. 3. (A & B) Levels of the IL8 and IL6 cytokines (in pg/mL) as analyzed through ELISA of the c-MSCs, uncoated MSCs, and c-MSCs and uncoated MSCs stimulated by IFNγ and TNFα. (C–G) mRNA expression levels of the il6, il8, vegf, tgfβ and hif1 in uncoated and c-MSCs (n = 6). Statistics done by Anova. *P < 0.05, **P < 0.01.

    Journal: Biomaterials advances

    Article Title: Biomimetic polyelectrolyte coating of stem cells suppresses thrombotic activation and enhances its survival and function.

    doi: 10.1016/j.bioadv.2023.213331

    Figure Lengend Snippet: Fig. 3. (A & B) Levels of the IL8 and IL6 cytokines (in pg/mL) as analyzed through ELISA of the c-MSCs, uncoated MSCs, and c-MSCs and uncoated MSCs stimulated by IFNγ and TNFα. (C–G) mRNA expression levels of the il6, il8, vegf, tgfβ and hif1 in uncoated and c-MSCs (n = 6). Statistics done by Anova. *P < 0.05, **P < 0.01.

    Article Snippet: For the protein level cytokine analysis of IL6 and IL8, human IL6 DuoSet ELISA kit (DY206) and human IL8 DuoSet ELISA kit (DY208) were obtained from R&D Systems (Biotechne).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing

    Fig. 4. Comparing the immunomodulatory properties of the c-MSCs and MSCs on stimulated cells. (A) The effect of the conditioned media (CM) from c-MSCs and MSCs on the LPS stimulated C28/I2 chondrocytes. Statistics done using Anova. *P < 0.05, ***P < 0.001 (n = 6) The effect of the CM of c-MSCs and MSCs on the M1 polarized THP1 cells was measured by analyzing the levels of (B) TNFα (C) IL1β (D) IP10 and (E) IL6.

    Journal: Biomaterials advances

    Article Title: Biomimetic polyelectrolyte coating of stem cells suppresses thrombotic activation and enhances its survival and function.

    doi: 10.1016/j.bioadv.2023.213331

    Figure Lengend Snippet: Fig. 4. Comparing the immunomodulatory properties of the c-MSCs and MSCs on stimulated cells. (A) The effect of the conditioned media (CM) from c-MSCs and MSCs on the LPS stimulated C28/I2 chondrocytes. Statistics done using Anova. *P < 0.05, ***P < 0.001 (n = 6) The effect of the CM of c-MSCs and MSCs on the M1 polarized THP1 cells was measured by analyzing the levels of (B) TNFα (C) IL1β (D) IP10 and (E) IL6.

    Article Snippet: For the protein level cytokine analysis of IL6 and IL8, human IL6 DuoSet ELISA kit (DY206) and human IL8 DuoSet ELISA kit (DY208) were obtained from R&D Systems (Biotechne).

    Techniques: